Figure 3: FUS1 reduces the ability of SCLC cells to form colonies. (A) DMS273 and DMS53 cells were co-transfected with an EV, wt-FUS1 or mt-FUS1 expressing vector and a neomycin-resistant-gene containing vector. The cells were re-plated immediately after transfection and grown for 3-6 weeks in growth medium containing G418. G418-resistant colonies were stained with Toluidin blue. (B) Quantification of the number of G418-resistant colonies relative to cells transfected with EV plasmid. The experiments were performed twice in duplicates. Bars indicate SEM.