Figure 4: Cell cycle analysis of transfected SCLC cells. (A) Cell cycle analysis was performed 72 h after transfection with either EV, wt-FUS1 or mt-FUS1 plasmid. The cells were fixated and stained with propidium Iodide after which they were analysed using a flow-cytometer. Representative histograms are shown. (B) Quantification of the Sub-G1/G0 population seen in figure (A). The experiments were performed at least 3 times. Bars indicate SEM and asterisks indicate statistical significance of the differences compared to the EV control transfectants as determined by student’s t-test (*P<0.05; ** P<0.001).