Drugs Conc. injected Inter-day Intra-day µgmL-1 %RSD %Rec %RSD %Rec CAP 2.5 0.0073 101.11 0.073 101.11 5 0.0109 102.36 0.009 102.36 10 0.3261 100 0.361 100.02 15 0.0009 100 0.09 100.03 20 0.0005 99.826 0.005 99.26 25 0.0002 99.998 0.002 99.98 HCT 2.5 0.0047 99.997 0.047 99.9 5 0.0071 99.988 0.071 99.88 10 0.0024 100.12 0.024 100.1 15 0.0006 99.983 0.006 99.93 20 0.0006 99.968 0.006 99.98 25 0.0003 99.991 0 99.91 FRS 2.5 0.0075 98.72 0.007 98.72 5 0.0075 99.98 0.075 99.98 10 0.0019 99.73 0.019 99.73 15 0.0012 99.97 0.012 99.87 20 0.0005 99.97 0.005 99.87 25 0.0003 100.02 0.003 100.1 CAP: captopril; HCT: hydrochlorothiazide; FRS: furosemide

Table 4: Inter and intra-day precision of the proposed method for captopril, hydrochlorothiazide and furosemide (n=6).