| |
Level |
K’ |
T |
(Rs) |
| A: pH of mobile phase |
| 2.8 |
-0.2 |
2.5 |
1.51 |
2.37 |
| 3 |
0 |
2.6 |
1.53 |
3 |
| 3.2 |
0.2 |
2.2 |
1.58 |
2.35 |
| S.D n=6 |
|
0.21 |
0.04 |
0.01 |
| B: Flow rate (mLmin-1) |
| 0.8 |
-0.2 |
2.1 |
1.56 |
2.32 |
| 1 |
0 |
2.6 |
1.53 |
3 |
| 1.2 |
0.2 |
2.5 |
1.51 |
2.37 |
| S.D n=6 |
|
0.28 |
0.03 |
0.03 |
| C: Percentage of water in mobile phase (V/V) |
| 90/10 |
-20 |
2.7 |
1.52 |
2.39 |
| 70/30 |
0 |
2.6 |
1.53 |
3 |
| 80/20 |
-10 |
2.4 |
1.57 |
2.32 |
| S.D n=6 |
|
0.21 |
0.03 |
0.04 |
| D: Wavelength (nm) |
| 220 |
-5 |
2.7 |
1.52 |
2.39 |
| 225 |
0 |
2.6 |
1.53 |
3 |
| 230 |
5 |
2.5 |
1.59 |
2.33 |
| S.D n=6 |
|
0.14 |
0.04 |
0.03 |
| K’= Capacity factors, T = Tailing factor, Rs = Resolution |
|
