| Parameter |
NEB |
HCTZ |
| S.D. of Peak Area |
% R.S.D. |
S.D. of Peak Area |
% R.S.D |
| Mobile Phase Composition |
| Toluene: ethyl acetate: methanol: ammonia
(3.2:2.9:1.9:0.1) |
67.14 |
1.78 |
145.80 |
1.25 |
| Toluene: ethyl acetate: methanol: ammonia
(2.8:2.5:1.5:0.1) |
50.08 |
1.34 |
133.44 |
1.16 |
| Mobile Phase Volume |
| 7.5 ml |
74.30 |
1.95 |
140.25 |
1.21 |
| 15 ml |
51.43 |
1.37 |
150.05 |
1.29 |
| Development distance |
| 8 cm |
69.38 |
1.84 |
128.76 |
1.11 |
| 8.5 cm |
50.17 |
1.33 |
64.20 |
0.56 |
| 9 cm |
39.37 |
1.01 |
105.09 |
0.90 |
| Duration of saturation |
|
| 20 min |
67.75 |
1.72 |
142.84 |
1.24 |
| 25 min |
54.69 |
1.38 |
65.65 |
0.56 |
| 30 min |
49.57 |
1.28 |
110.06 |
0.95 |
| Activation of prewashed plates |
| 5 min |
76.97 |
1.98 |
193.34 |
1.66 |
| 10 min |
64.52 |
1.71 |
197.03 |
1.70 |
| 15 min |
61.81 |
1.69 |
161.87 |
1.39 |
| Time from spotting to chromatography |
| 30 min |
65.71 |
1.75 |
119.17 |
1.02 |
| 60 min |
63.80 |
1.70 |
170.42 |
1.47 |
| Time from chromatography to scanning |
| 30 min |
47.48 |
1.25 |
131.04 |
1.12 |
| 60 min |
71.68 |
1.91 |
145.70 |
1.25 |
