Table 1

Ratio (Acetonitrile:methanol:buffer)	*D_m*	*R_r*	R	T
10:10:80	1.41	2.41	10.73	1.40
15:15:70	0.86	1.86	8.38	1.23
20:20:60	0.66	1.66	8.73	1.07
25:25:50	0.57	1.57	5.65	1.30
30:30:40	0.57	1.57	4.36	1.33

D_m is the mass distribution ratio [also known as the capacity factor (K`) or the retention factor (K)].
R_r is the relative retention times [also known as the unadjusted relative retention (r_G)].
R is the resolution.
T is the tailing factor [also known as the symmetry factor (A_s)].

Table 1: Effect of the ratio of mobile phase components on the chromatographic performance of the HPLC method