Concentration of ethanol(%,w/w) Volume of eluent(mL) Content of anthocyanins(mg) DR (%)
0 59 --- ---
20 60 --- ---
30 63 0.012 0.015
40 41 0.65 0.84
60 205 75.97 98.30
80 84 0.65 0.84
Table 2: Gradient desorption result of anthocyanins.