| Parameter |
Description |
| Column name |
Kromasil 100 C–18 (50 × 2.1 mm), 3.5 μ |
| Mobile phase* |
Acetonitrile: TEA buffer (0.1%TEA, pH 3±0.05 with OPA) |
| Flow rate |
0.8 mL/min |
| Weak Wash |
9:1 (MQ water: Acetonitrile) |
| Strong Wash |
1:9 (MQ water: Acetonitrile) |
| Detection |
UV at 240 nm |
| Column Temperature |
40°C |
| Sample Temperature |
25°C |
| Injection volume |
2 μL |
| Run time |
2.2 min |
|
