| Parameter |
Data |
| Linearity range( ng per spot) |
500–3000 |
| Limit of Detection ( ng ) |
30 |
| Limit of Quantitation ( ng ) |
82 |
| Accuracy (n = 9) |
99.54±0.42 |
| Precision (% RSD) Repeatability of application (n = 7) |
1.65 |
| Interday (n = 3) |
0.49 |
| Intraday (n = 3) |
0.78 |
| Specificity |
Specific |
| Robustness |
Robust |
*mean of three estimations |