| Parameter |
S.D. of peak area |
%R.S.D. |
| Mobile phase composition |
92.32 |
0.33 |
| Amount of mobile phase |
60.44 |
0.92 |
| Temperature |
71.08 |
0.62 |
| Relative humidity |
81.02 |
1.28 |
| Plate pretreatment |
84.25 |
1.27 |
| Time from spotting to chromatography |
96.51 |
1.61 |
| Time from chromatography to scanning |
88.31 |
1.29 |
|