Method validation parameters D1 DD1 TLC HPLC Linearity Slope Intercept Correlation coefficient(r) 0.0248 0.0011 0.9999 0.2499 0.0116 0.9997 1468.6 82.332 0.9998 39.38 -10.248 0.9998 Linearity range (μg/ml) 1.6-22.40 1.6-22.40 0.50-4.50 (μg/spot) 10-120 Working range (μg/ml) Ringbom 5.00-20.00 5.00-20.00 2.0-4.50 (μg/spot) 15.00-117 Accuracy 99.55 ± 1.051 100.95 ± 0.713 99.68 ± 1.074 100.51 ± 1.460 Standard error of slope 0.001 0.002 18.201 10.201 Confidence limit of slope 0.026-0.013 0.024-0.012 287.945-276.872 37.257-36.893 Precision Intraday Interday 99.99 ± 0.254 100.42 ± 0.870 98.45 ± 0.147 100.64 ± 0.780 99.35 ± 0.494 99.73 ± 0.530 98.75 ± 0.904 101.93 ± 0.480 L.O.D L.O.Q 0.12 0.70 0.15 0.69 0.05 0.15 0.06 0.13 Intraday (n=3) average of three different concentration repeated three times within a day. Interday ( n=3) average of three different concentration repeated three times in successive days. Ringbom % absorbence against logarithim of concentration. L.O.D limit of detection, L.O.Q limit of quantitation.

Table 2: Characteristic parameters of rivaroxaban by the proposed methods.