Parameters Timolol maleate Bimatoprost Timolol maleate degradate Bimatoprost degradate Reference values
Resolution 5.70 4.90 >1.5
Tailing factor (T) 1.20 1.40 1.10 1.00 <1.5
Capacity factor (K) 1.83 6.22 8.26 8.25 >1
Selectivity (α) 3.40 1.33 1.30 1-10
Theoretical plates(N) 12641 11315 14532 10248 >3000
Retension time (min.) 2.539 ± 0.01  7.224 ± 0.01 9.269 ± 0.01 13.892 ± 0.02 _
HETP 0.0019 0.0022 0.0017 0.0024 Smaller the no., higher the efficiency
Table 1: System suitability parameters for determination of bimatoprost, timolol maleate and their alkaline degradates by HPLC proposed method.