Mixture number BIM TIM BIM TIM Taken (μg/ml) Taken (μg/ml) Found* (µg/ml) Recovery% Found* (μg/ml) Recovery% Degradate Intact drug Degradate Intact drug 1 3 30 10 70 29.95 99.83 70.70 101.00 2 5 20 10 60 19.68 98.40 59.75 99.58 3 10 10 20 40 10.01 100.01 39.20 98.01 4 20 10 50 20 10.12 101.24 19.97 99.87 Mean±RSD%   99.87 ± 1.015   99.52 ± 1.093 Average of three determination

Table 3: Determination of bimatoprost and timolol maleate in presence of their alkaline degradates in laboratory proposed mixture by proposed HPLC method.