|
Parameter |
Bimatoprost |
Manufacturer’s
HPLC Method |
Timolol
maleate |
Official HPLC
Method |
| Mean |
100.84 |
100.04 |
99.67 |
100.19 |
| SD |
0.644 |
0.776 |
0.912 |
1.074 |
| RSD% |
0.639 |
0.776 |
0.915 |
1.072 |
| n |
7 |
5 |
7 |
5 |
| Variance |
0.41 |
0.60 |
0.83 |
1.15 |
| Student’s test (2.22) |
0.11 |
|
0.69 |
|
|
F Value (5.43) |
1.46 |
|
1.39 |
|
HPLC method using C18 column, mobile phase water: acetonitrile: methanol
(72:18:10, by volume), flow rate 1.5 ml/min and detection was at λ210 nm. BIM |
