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Figure 1: 10% Native PAGE of a protein extract from tissue fluids of the mussel Isognomomalatus: lane 1 molecular weight (MW) marker (Sigma-Aldrich Co); lane 2 protein extract with MW of 220 kDa and lane 3 western blotting confirmation of the protein extract presence and MW of the proteins in the tissue fluids of the mussel I. alatus. The western blotting used an anti-Isognomonalatus tissue fluid protein extract developed in rats and a peroxidase- labeled protein LA conjugate (Sigma-Aldrich Co) as tools. |