Method of IgY Separation IgY Protein concentration Titer of anti-salmonella Ab Laying hen Ostrich Laying he Ostrich TCA method 200-250 mg/egg yolk 4200-5000 mg/egg yolk 1: 256 1: 128 PEG method 100-130 mg/egg yolk 2000-2200 mg/egg yolk 1: 64 1: 32

Table 1: IgY concentration and Anti-salmonella typhimurium titer of Laying hen and Ostrich in different Separation techniques.