Figure 1: C-peptide detection with two collision dissociation reactions 1007.7-147.2 and 1007.7-927.8. Panels A and B correspond to each single reaction. In panel C, the product of the two mass-spectrograms is given. A relatively high sample load (30 pg) provides for strong signals both for 1007.7-147.2 and 1007.7-927.8 transitions. An excellent signal for the product function is also obtained. MS conditions: positive ionization mode. The transition reactions are for the triple ionized parent C-peptide ion [3].