| Parameter |
Optimized condition |
| Chromatograph |
HPLC with UV- detector |
| Column |
C8 Column |
| Mobile Phase |
pH-4.2 phosphate buffer in the ratio and methanol of 70:30(v/v) |
| Flow rate |
1.00ml/min |
| Detection |
298nm |
| Injection volume |
10μl. |
| Temperature column |
Room temperature |
|
