Figure 5: A direct ELISA was performed with serum samples 1, 2, 3, fraction no. 2 from serum samples 1, 2 and 3. Individual antigens were coated in to micro titer ELISA plates at a concentration of 10 μg/ml in 0.1M sodium carbonate and bicarbonate buffer. After coating, wells were washed with rinse buffer. Unadsorbed sites were blocked with blocking buffer .Excess blocking buffer was removed by washing with rinse buffer. After blocking, the wells were treated with anti-human IgG -HRP conjugate. Excess labeled antibody was removed by washing the wells with rinse buffer and finally TMB/H2O2 was added in to each well. As negative controls rabbit IgG were coated in few wells. Results of such an experiment are shown in the figure.