A1A2-Antibody Control; A3A4-Rabbit IgG (Negative control)
B1B2-Serum sample-1; B3B4-Serum sample -2;
C1C2-Serum sample-3; C3C4-Fraction no. 2 (Serum sample 1);
D1D2-Fraction no. 2 (Serum sample 2); D3D4-Fraction no 2 (Serum sample 3)
Figure 12: Enzyme Linked Immunosorbent Assay.