Ingredients
7.5%
10%
12%
15%
1
1.5M Tris HCl pH 8.8 (ml)
2.5
2.5
2.5
2.5
2
Acrylamide: Bisacrylamide
2.5
3.33
4.0
5.0
3
Distilled water
4.83
4.0
3.33
2.33
4
10% SDS
0.1
0.1
0.1
0.1
5
TEMED
10
10
10
10
6
10% APS
75
75
75
75
Table 3:
Preparation of Separating Gel (10 ml).
