Ingredients
1
0.5M Tris Hel pH 6.8
0.75 ml
2
Acrylamide-Bisacrylamide
1.0 ml
3
Distilled water
3.15 ml
4
10% SDS
50 μl
5
10% APS
45 μl
6
TEMED
10 μl
Table 4:
Preparation of Stacking Gel (5 ml).