Ingredients
1 0.5M Tris Hel pH 6.8 0.75 ml
2 Acrylamide-Bisacrylamide 1.0 ml
3 Distilled water 3.15 ml
4 10% SDS 50 μl
5 10%  APS 45 μl
6 TEMED 10 μl
Table 4: Preparation of Stacking Gel (5 ml).