| Formulation For Preparation Of Different Percentages Of Separating Gel (10ml) |
| |
Ingredients |
7.5 % |
10 % |
12 % |
15 % |
| 1 |
1.5M Tris HCl pH 8.8 (ml) |
2.5 ml |
2.5 ml |
2.5 ml |
2.5 ml |
| 2 |
Acrylamide: Bisacrylamide |
2.5 ml |
3.33 ml |
4.0 ml |
5.0 ml |
| 3 |
Distilled water |
4.93 ml |
4.10 ml |
3.43 ml |
2.43 ml |
| 4 |
TEMED |
10 μl |
10 μl |
10 μl |
10 μl |
| 5 |
10% APS |
75 μl |
75 μl |
75 μl |
75 μl |
|
