| Factor |
Units |
Limits |
Level(-1) |
Level (+1) |
Nominal(0) |
| A. pH phosphate buffer |
|
± 0.5 |
6.5 |
7.5 |
7 |
| B. Buffer concentration |
mM |
± 2 |
3 |
7 |
5 |
| C. Flow rate |
mL min-1 |
± 0.2 |
1.2 |
0.8 |
1.00 |
| D. Initial % solvent B (step1) |
% |
± 2 |
18 |
22 |
20 |
| E. First gradient program time (step 2) |
Min |
± 0.2 |
0.8 |
1.2 |
1 |
| F. % solvent B (step 3) |
% |
± 2 |
38 |
42 |
40 |
| G. Second held time (step 3) |
Min |
± 1 |
9 |
11 |
10 |
| |
|
|
|
|
|
Level 0: optimal values
Level +1: upper values
Level -1: lower values |
