Parameters BEN HCT
Limit of Detection 0.065 0.0145
Limit of Quantitation 0.1 0.5
Accuracy (%) 98.66 - 99.32 98.93-99.73
Repeatability (%RSD, n = 6) 0.30-1.68 0.20-1.43
Precision    
Intraday (n = 3) 0.11-0.19 0.11-0.48
Interday (n = 3) 1.11-1.72 1.22-1.70
Specificity Specific Specific
Robustness Robust Robust
Solvent suitability Suitable for 24hrs Suitable for 24hrs
Table 2: Summary of validation Parameters of RP-HPLC.