| Method parameter |
Normal condition |
Deliberate changes |
% RSD of peak area (n=3) |
| BEN |
HCT |
| Flow rate |
1.5 ml/min |
0.8 ml/min |
0.95 |
0.84 |
| 1.2ml/min |
0.68 |
0.71 |
| Mobile phase ratio |
Water:Methanol(55:45) |
53:47 |
0.82 |
0.29 |
| 57:43 |
0.56 |
0.63 |
| pH of mobile phase ratio |
7 |
7.2 |
0.92 |
0.81 |
| 6.8 |
0.65 |
0.76 |
|
