Parameters TLC densitometric method [n=11] HPLC method [n=10] Linearity range 0.100 – 2.00 μg per spot 2 – 40 μg.ml-1 Correlation coefficient 0.9996 0.9998 Detection limit (LOD) 0.033 0.593 Quantitation limit (LOQ) 0.100 1.790 Slope ± SD 1902.5 ± 13.555 3.4813 ± 0.0275 Confidence limit of slopea 1900.3 – 1905.7 3.461 – 3.500 S.E. of slope 4.087 0.0087 Intercept ± SD 86.803 ± 19.025 0.2747 ± 0.626 Confidence limit of intercepta 85.765- 87.435 -0.183 – 0.732 S.E. of intercept 5.736 0.198 a: 95% confidence limit

Table 1: Linear regression data for the calibration curves.