TLC densitometric methoda HPLC methoda Intra-day   Concentration μg per spot Recovery% SD %R.S.D. Concentration μg.ml-1 Recovery% SD %R.S.D. 0.300 100.667 0.004 1.325 6.000 100.500 0.065 1.078 1.200 99.667 0.031 2.592 20.000 99.450 0.211 1.061 2.000 99.250 0.042 2.116 35.000 100.886 0.432 1.223 Inter-day   1st day 99.000 0.005 1.684   1st day 99.670 0.068 1.137 0.300 2nd day 101.333 0.004 1.316 6.000 2nd day 98.500 0.072 1.218   3rd day 99.667 0.006 2.007   3rd day 98.000 0.075 1.276   1st day 100.917 0.032 2.642   1st day 99.550 0.266 1.336 1.200 2nd day 100.500 0.027 2.239 20.000 2nd day 100.550 0.301 1.497   3rd day 99.083 0.029 2.439   3rd day 99.850 0.289 1.447   1st day 97.700 0.042 2.149   1st day 98.770 0.511 1.478 2.000 2nd day 101.050 0.038 1.880 35.000 2nd day 100.340 0.587 1.671   3rd day 102.250 0.045 2.200   3rd day 98.91 0.603 1.742 aEach result is an average of three determinations.

Table 2: Accuracy and precision of TLC densitometric and HPLC methods.