| |
Parameters |
S.D.[peak area) |
% R.S.D. |
S.E. |
| TLC densitometric method |
Mobile phase composition |
0.415 |
0.021 |
0.169 |
| Mobile phase volume [± 10%] |
0.799 |
0.040 |
0.326 |
| Time of saturation [± 10 minutes] |
0.225 |
0.011 |
0.092 |
| Time from spotting to development [±10 min] |
0.309 |
0.015 |
0.126 |
| Time from chromatography to scanning [±10 min] |
0.319 |
0.016 |
0.130 |
| HPLC method |
Change in organic phase percent in mobile phase [±3.00%] |
0.383 |
0.542 |
0.156 |
| Change in mobile phase pH [± 0.20] |
0.187 |
0.265 |
0.076 |
| Change in wavelength of detection [± 2.00] |
0.137 |
0.194 |
0.056 |
| Change in flow rate [± 0.1] |
0.375 |
0.531 |
0.153 |
| Change column supplier |
0.467 |
0.661 |
0.191 |
