TLC densitometric method HPLC method Added standard (μg per spot) Recovery % Added standard (μg/ml) Recovery % 0.2 100.450 5.000 101.740 0.6 98.717 10.000 99.210 1 99.650 20.000 99.490 1.4 99.114 30.000 100.040 1.8 99.500 40.000 99.160

Table 5: Application of standard addition technique on pazufloxacin IV injection using TLC densitometric and HPLC methods [n=3].