Figure 2: Proteolysis of syn1-99, syn1-52, syn57-102 and syn108-140 by proteinase K analyzed by RP-HPLC. The reactions were conducted at a protein concentration of 5 μM in PBS buffer in the absence (top) or in the presence (bottom) of 250 μM DHA, using an E/S ratio of 1:1000. RP-HPLC chromatograms correspond to the analysis of aliquots taken from the proteolysis mixtures after 1-hr of incubation with the protease.