Method
Gemifloxacin mesylate
Theophylline
Warfarin
Omeprazole
RP-HPLC
Concentration (µg/ml)
SD (n=5)
CV %
% RE
Concentration (µg/ml)
SD (n=5)
CV %
% RE
Concentration (µg/ml)
SD (n=5)
CV %
% RE
Concentration (µg/ml)
SD (n=5)
CV %
% RE
intraday
0.05
0.0025
5.187
-3.60
0.25
0.0043
1.748
-1.6
0.1
0.0054
5.625
-4
0.1
0.0047
4.947
-5
0.15
0.004
2.400
0.00
0.75
0.005
0.668
-0.267
0.3
0.007
2.034
-1.667
0.3
0.006
1.993
0.333
1
0.012
1.206
-0.50
4
0.054
1.343
0.55
2
0.025
1.272
-1.7
1
0.012
1.268
-0.7
5
0.052
1.041
0.30
7
0.112
1.603
-0.2
9
0.204
2.262
0.222
4
0.084
2.087
0.625
interday
0.05
0.0023
4.591
0.20
0.25
0.004
1.639
-2.4
0.1
0.006
6.122
-2
0.1
0.0051
5.414
-5.8
0.15
0.003
2.000
0.00
0.75
0.006
0.799
0.133
0.3
0.005
1.672
-0.333
0.3
0.006
2.007
-0.333
1
0.031
3.054
1.50
4
0.090
2.264
-0.6
2
0.036
1.769
1.75
1
0.020
1.972
1.4
5
0.078
1.570
-0.70
7
0.218
3.061
1.729
9
0.196
2.165
0.578
4
0.132
3.313
-0.4
TLC intraday
0.1
0.006
6.186
-3
0.5
0.032
6.695
-4.4
0.2
0.006
3.125
-4
0.1
0.002
2.083
-4
0.3
0.013
4.498
-3.667
1.5
0.058
3.862
0.133
0.6
0.016
2.658
0.333
0.3
0.004
1.408
-5.333
1.5
0.026
1.751
-1
3
0.084
2.953
-5.167
1
0.021
2.176
-3.5
0.8
0.013
1.656
-1.875
2.5
0.062
2.545
-2.56
5
0.133
2.717
-2.08
2
0.092
4.725
-2.65
1.2
0.043
3.720
-3.667
interday
0.1
0.005
4.990
0.2
0.5
0.026
5.556
-6.4
0.2
0.009
4.839
-7
0.1
0.004
4.301
-7
0.3
0.015
5.119
-2.333
1.5
0.061
4.144
-1.867
0.6
0.023
3.879
-1.167
0.3
0.005
1.779
-6.333
1.5
0.030
2.009
-0.467
3
0.092
3.273
-6.3
1
0.033
3.290
0.3
0.8
0.016
2.062
-3
2.5
0.046
1.849
-0.48
5
0.162
3.401
-4.72
2
0.102
5.380
-5.2
1.2
0.052
4.384
-1.167
CV %: coefficient of variation
% RE: percent relative error
Table 1:
Accuracy and precision of gemifloxacin mesylate, Theophylline, Warfarin and Omeprazole in human plasma.
