Gemifloxacin
Theophylline
Warfarin
Omeprazole
Added plasma concentration (µg/ml)
Found* (Mean± SD)
CV%
Added plasma concentration (µg/ml)
Found* (Mean± SD)
CV%
Added plasma concentration (µg/ml)
Found* (Mean± SD)
CV%
Added plasma concentration (µg/ml)
Found* (Mean± SD)
CV%
Freeze-thaw
0.05
0.0502± 0.003
6.374
0.25
0.243± 0.005
1.893
0.1
0.098± 0.005
5.306
0.1
0.094± 0.006
6.064
0.15
0.1489± 0.003
1.746
0.75
0.7493± 0.006
0.774
0.3
0.3011± 0.005
1.594
0.3
0.2966± 0.006
1.922
1
1.003± 0.023
2.333
4
3.965± 0.069
1.735
2
1.969± 0.036
1.854
1
0.9885± 0.021
2.165
5
5.035± 0.114
2.264
7
7.115± 0.174
2.446
9
8.885± 0.205
2.307
4
4.114± 0.132
3.209
Short term
0.05
0.0485± 0.003
5.979
0.25
0.2504± 0.004
1.518
0.1
0.101± 0.005
4.752
0.1
0.098± 0.005
5.408
0.15
0.1503± 0.004
2.728
0.75
0.752± 0.007
0.904
0.3
0.312± 0.004
1.346
0.3
0.3025± 0.004
1.190
1
1.008± 0.034
3.383
4
4.088± 0.091
2.228
2
2.052± 0.064
3.099
1
1.013± 0.019
1.935
5
5.113± 0.201
3.931
7
7.066± 0.217
3.071
9
9.032± 0.265
2.934
4
4.052± 0.196
4.837
Long term
0.05
0.0492± 0.003
7.114
0.25
0.246± 0.004
1.707
0.1
0.095± 0.005
6.000
0.1
0.095± 0.005
5.895
0.15
0.1496± 0.005
3.208
0.75
0.748± 0.009
1.229
0.3
0.298± 0.005
1.778
0.3
0.2963± 0.007
2.396
1
0.989± 0.029
2.963
4
3.966± 0.078
1.979
2
1.974± 0.071
3.602
1
0.9932± 0.023
2.366
5
4.989± 0.236
4.730
7
6.982± 0.306
4.383
9
8.963± 0.301
3.358
4
3.942± 0.223
5.657
Post preparative
0.05
0.0501± 0.003
6.786
0.25
0.247± 0.004
1.781
0.1
0.097± 0.005
5.567
0.1
0.101± 0.006
6.039
0.15
0.1501± 0.004
2.798
0.75
0.752± 0.006
0.838
0.3
0.305± 0.006
1.869
0.3
0.301± 0.004
1.194
1
0.985± 0.028
2.883
4
4.011± 0.0902
2.249
2
1.996± 0.046
2.305
1
1.011± 0.019
1.939
5
4.988± 0.102
2.049
7
7.062± 0.141
1.997
9
9.05± 0.185
2.044
4
4.023± 0.096
2.399
• Mean of five determinations
Table 3:
Stability study of gemifloxacin, theophylline, warfarin and omeprazole in human plasma by the proposed HPLC method.
