Gemifloxacin
Theophylline
Warfarin
Omeprazole
Added plasma concentration (µg/ml)
Found* (Mean± SD)
CV%
Added plasma concentration (µg/ml)
Found* (Mean± SD)
CV%
Added plasma concentration (µg/ml)
Found* (Mean± SD)
CV%
Added plasma concentration (µg/ml)
Found* (Mean± SD)
CV%
Freeze-thaw
0.1
0.095± 0.006
6.316
0.5
0.477± 0.032
6.709
0.2
0.187± 0.006
3.209
0.1
0.093± 0.003
3.226
0.3
0.285± 0.016
5.614
1.5
1.395± 0.056
4.014
0.6
0.574± 0.013
2.265
0.3
0.283± 0.005
1.767
1.5
1.442± 0.032
2.219
3
2.805± 0.092
3.280
1
0.936± 0.046
4.915
0.8
0.781± 0.016
2.049
2.5
2.415± 0.062
2.567
5
4.855± 0.202
4.161
2
1.885± 0.105
5.570
1.2
1.174± 0.036
3.066
Short term
0.1
0.097± 0.006
6.186
0.5
0.489± 0.026
5.317
0.2
0.192± 0.005
2.604
0.1
0.096± 0.003
3.125
0.3
0.302± 0.014
4.636
1.5
1.450± 0.062
4.276
0.6
0.591± 0.018
3.046
0.3
0.291± 0.007
2.405
1.5
1.486± 0.029
1.952
3
2.936± 0.102
3.474
1
0.942± 0.032
3.397
0.8
0.792± 0.017
2.146
2.5
2.501± 0.058
2.319
5
4.962± 0.165
3.325
2
1.925± 0.101
5.247
1.2
1.184± 0.028
2.365
Long term
0.1
0.096± 0.005
5.208
0.5
0.478± 0.029
6.067
0.2
0.182± 0.008
4.396
0.1
0.094± 0.002
2.128
0.3
0.281± 0.018
6.406
1.5
1.433± 0.081
5.652
0.6
0.584± 0.016
2.740
0.3
0.286± 0.009
3.147
1.5
1.466± 0.036
2.456
3
2.856± 0.122
4.272
1
0.926± 0.062
6.695
0.8
0.783± 0.018
2.299
2.5
2.425± 0.048
1.979
5
4.725± 0.205
4.339
2
1.900± 0.092
4.842
1.2
1.173± 0.034
2.899
Post preparative
0.1
0.101± 0.007
6.931
0.5
0.493± 0.021
4.260
0.2
0.196± 0.009
4.592
0.1
0.097± 0.004
4.124
0.3
0.301± 0.011
3.654
1.5
1.485± 0.055
3.704
0.6
0.592± 0.011
1.858
0.3
0.289± 0.011
3.806
1.5
1.495± 0.034
2.274
3
2.896± 0.065
2.244
1
0.965± 0.052
5.389
0.8
0.766± 0.025
3.264
2.5
2.496± 0.006
2.644
5
4.905± 0.094
1.916
2
1.922± 0.102
5.307
1.2
1.154± 0.041
3.553
• Mean of five determinations
Table 4:
Stability study of gemifloxacin, theophylline, warfarin and omeprazole in human plasma by the proposed TLC method.
