Parameter Gemifloxacin Theophylline Warfarin Omeprazole HPLC TLC HPLC TLC HPLC TLC HPLC TLC Capacity factor (K') 3.56 4.16 2.67 5.44 4.35 4.07 3.66 3.17 Selectivity factor (α) 1.44 1.08 1.36 1.24 1.76 1.56 1.08 1.56 Resolution (R) 3.83 2.65 2.08 1.78 2.55 1.98 4.55 3.78 Theoretical plates (N) 3589.56 2654.77 4554.76 2564.78 3786.88 3007.78 5641.67 3211.67 Tailing factor (t) 1.33 1.45 1.15 1.55 1.25 1.33 1.03 1.15

Table 7: System suitability results of the HPLC and TLC methods for determination gemifloxacin, theophylline, warfarin and omeprazole in spiked human plasma.