Parameter OMP TND CLR Limit (49,50)
Retention time (tR) 2.063 1.353 5.443  
Resolution (RS) 3.95 R S> 2
Tailing factor (T) 0.80 0.88 0.40 T = 1 for a typical symmetric peak
Capacity factor  (K’) 1.58 1.48 5.06 1 -10 acceptable
Selectivity factor (α) 3.27 α> 1
Column efficiency (N) 9811 6991 2451 N > 2000
Height equivalent to theoretical plate (HETP) 0.003 0.004 0.010 The smaller the value, the higher the column efficiency
Table 7: System suitability parameters of the proposed HPLC method.