Sample Putrescine Spermidine Spermine # H2O Borate H2O Borate H2O Borate 1 14.41 14.05 33.63 33.80 30.56 31.71 2 18.69 17.99 66.55 62.72 51.59 56.02 3 20.42 21.00 26.73 25.88 44.88 44.83 4 7.66 8.01 14.10 15.01 56.50 56.77 5 10.58 10.44 17.22 17.07 55.75 56.03 6 29.07 30.05 57.54 55.66 79.03 78.77 7 40.09 42.88 26.64 26.72 43.33 43.63 8 26.04 26.33 32.91 30.88 45.61 46.04 9 27.92 27.67 16.66 17.78 58.08 59.00 10 37.57 39.32 12.90 13.41 46.21 46.08 11 29.47 29.53 29.01 28.95 59.78 60.01 12 34.34 32.44 26.92 24.65 53.69 52.33 13 58.57 57.99 18.88 17.89 45.45 43.85 14 26.56 24.65 14.57 14.87 54.34 54.44 15 9.45 9.80 80.91 81.04 61.56 61.78 Average 26.06 26.14 31.68 31.09 52.42 52.75 Sample Sd 13.51 13.68 20.61 19.97 10.90 10.85 Sd by OPA group 0.12 0.45 0.03

Table 2: Concentration of plasma polyamine (amine group) in pmol/ml, using o-phthaldialdeyde with H2O and borate.Sd by sample group and Sd by OPA group ( Water x Borate).