Putrescine Spermidine Spermine
  H2O Borate H2O Borate H2O Borate
Means of means 21.6 21.2 50.4 50.3 62.0 62.4
Standard deviation 3.4 3.1 6.8 6.6 4.5 5.4
n 15 15 16 16 16 16
95% Confidence interval lower limit 19.7 19.5 46.8 46.8 59.6 59.5
95% Confidence  interval upper limit 23.5 22.9 54.0 53.9 64.3 65.3
Coefficient of variability (CV)% 15.8 14.6 13.5 13.2 7.2 8.6
Inter-assay CV 15.2 13.3 7.9
Intra-assay CV 5.9 2.5 1.8
Limit of quantification, pmol 0.20 0.22 15 13 20 20
Limit of detection, pmol 0.10 0.11 8 6 12 12
Table 3:Inter-assay and intra-day precision for polyamine (amine group) determination with water and borate at pH 10.4