Item Amlodipine besylate Losartan potassium Valsartan Atorvastatin calcium Linear range(µg/ml) 2-12 10-60 16-96 4-24 Detection limit (µg/ml) 0.03 0.18 0.15 0.007 Quantitation limit (µg/ml) 0.1 0.54 0.45 0.024 Regression data         N 5 5 5 5 Slope (b) 64.62 75.39 64.49 70.96 Standard deviation of the slope 0.71 0.1 0.065 0.69 Intercept (a) 6.0 8.39 30.19 33.99 Standard deviation of the intercept 0.62 4.09 2.95 0.17 Correlation coefficient ® 0.9998 0.9997 0.9998 0.9998 Standard error of regression 0.07 0.40 0.47 0.17 (Y = a + bC, where C is the concentration of the compound (μg/ml) and Y is the drug peak area

Table 4: Calibration data was resulted from method validation of Amlodipine besylate, Losartan potassium, Valsartan and Atorvastatin calcium respectively.