| Run |
Design |
Response |
| Coded factorsa |
Assay (%) |
| Factor – A Flow rate (μLmin-1) |
Factor – B Acenonitrile (%) |
Factor – C pH |
NBL |
VST |
| 1 |
-1 |
-1 |
1 |
98.23 |
98.95 |
| 2 |
-1 |
1 |
-1 |
99.56 |
99.61 |
| 3 |
-1 |
-1 |
-1 |
99.52 |
99.54 |
| 4 |
0 |
0 |
0 |
99.89 |
98.73 |
| 5 |
1 |
-1 |
-1 |
100.56 |
99.52 |
| 6 |
1 |
1 |
-1 |
100.16 |
98.56 |
| 7 |
1 |
1 |
1 |
99.61 |
98.93 |
| 8 |
0 |
0 |
0 |
99.28 |
99.86 |
| 9 |
-1 |
1 |
1 |
99.47 |
99.85 |
| 10 |
0 |
0 |
0 |
98.66 |
98.23 |
| 11 |
1 |
-1 |
1 |
98.36 |
99.56 |
*Randomized.
aThe low, middle (central) and high level of the factors were designated as (-1), (0)
and (1), respectively. |
