Concentration
(5-Fluorouracil) (ng ml
-1
)
Area (mV)
Concentration
(Leucovorin)
(ng ml
-1
)
Area (mV)
12.5
0.223
25
0.428
25.0
0.296
50
0.718
50.0
0.429
100
1.285
100.0
0.649
125
1.526
125.0
0.765
250
2.683
250.0
1.276
500
4.913
500.0
2.313
1000
9.214
Table 1:
Peak area of different conc. of 5-FU and LV for calibration curve in mobile phase.
