Replicate 1 Replicate 2 Combined Peptides 44742 43931 50672 Proteins (1 unique peptide) 5884 5846 6150 Proteins (2 peptides*) 5072 5036 5834

In the combined column are reported the numbers of protein and peptides identified at least in one of the two runs. (*at least one unique peptide)

Table 1: Protein and peptide identification after in solution IEF of 500μg of HEK cells peptides.