155 min gradient 240 min gradient Combined Peptides 17335 19115 22353 Proteins (1 unique peptide) 3276 3579 3782 Proteins (2 peptides*) 2525 2778 3384

In the combined column are reported the numbers of protein and peptides identified at least in one of the two runs. (*at least one unique peptide)

Table 2: Protein and peptide identification after in solution IEF of 50μg of HEK cells peptides.