Standard concentration (µg/ml) Inter day (n=5) Mean height ± SD         (% CV) Intra day (n=5) Mean height ± SD (% CV) 0.78 305.8 ± 32.78 (10.7) 368 ± 39.76 (10.8) 1.56 564.8 ± 43.1 (7.6) 725.2 ± 72.5 (9.9) 3.125 1587.6 ± 174.4 (10.9) 1645.4 ± 177.7 (10.7) 6.25 3481.6 ± 299 (8.5) 3535 ± 356.9 (10.0) 12.5 7747.2 ± 499.9 (6.4) 7955.8 ± 321.5 (4.04) 25 15030.2 ± 855.7 (5.6) 15537.8 ± 1049.5 (6.75) 50 34021.8 ± 1702 (5.0) 32011.6 ± 1974.2 (6.16)

All concentrations in μg/ml. SD- standard deviation, CV- coefficient of variation

Table 1: Linearity and reproducibility of plasma alpha-LA standards