Parameter Acetylbarlerin Barlerin Shanzhiside methyl ester
Limit of detection (LOD)
(µg ±SD)
0.07 ±3.10 0.05 ±2.69 0.05 ±2.08
Limit of quantification (LOQ)
(µg ±SD)
0.21 ±1.5 0.15 ±2.8 0.15 ±3.1
Linearity range (µg/spot) 1.42 – 4.95 0.28 – 1.67 0.60 – 3.60
r2 0.997 0.995 0.992
Slope ±SD 0.98 ±0.013 2.29 ±0.04 0.96 ±0.021
95 % Confidence interval of  slope 0.956 - 1.009 2.209 - 2.368 0.917 - 1.007
Intercept ±SD -203.14 ±42.7 249.52 ±40.5 53.82 ±49.89
95 % Confidence interval of  intercept -293.60- (-112.68) 163.73 - 335.32 -51.94 - 159.58
Table 1: Limit of detection, quantification and linear regression data for the calibration curves of shanzhiside esters