| Parameter | Acetylbarlerin | Barlerin | Shanzhiside    methyl ester | 
      
        | Limit    of detection (LOD) (µg ±SD)
 | 0.07    ±3.10 | 0.05    ±2.69 | 0.05    ±2.08 | 
      
        | Limit    of quantification (LOQ) (µg ±SD)
 | 0.21    ±1.5 | 0.15    ±2.8 | 0.15    ±3.1 | 
      
        | Linearity    range (µg/spot) | 1.42    – 4.95 | 0.28    – 1.67 | 0.60    – 3.60 | 
      
        | r2 | 0.997 | 0.995 | 0.992 | 
      
        | Slope    ±SD | 0.98    ±0.013 | 2.29    ±0.04 | 0.96    ±0.021 | 
      
        | 95    % Confidence interval of  slope | 0.956    - 1.009 | 2.209    - 2.368 | 0.917    - 1.007 | 
      
        | Intercept    ±SD | -203.14    ±42.7 | 249.52    ±40.5 | 53.82    ±49.89 | 
      
        | 95    % Confidence interval of  intercept | -293.60-    (-112.68) | 163.73    - 335.32 | -51.94    - 159.58 |