| Parameter |
Amount
spotted
(µg) |
Average amount
detected
(µg ± SD) |
% RSD |
| (A) Acetylbarlerin |
| Mobile phase composition 3:3.1:2.9:1 |
2.12 |
2.13 ± 51 |
2.39 |
| Mobile phase composition 3:3:3:1 |
2.12 ± 43 |
2.01 |
| Mobile phase composition 3.2:2.8:3:1 |
2.12 ± 51 |
2.40 |
| Mobile phase volume 8 ml |
2.12 ± 54 |
2.53 |
| Mobile phase volume 10 ml |
2.12 ± 43 |
2.01 |
| Mobile phase volume 12 ml |
2.13 ± 39 |
1.86 |
| Chamber saturation time: 5 min |
2.12 ± 57 |
2.69 |
| Chamber saturation time: 10 min |
2.12 ± 43 |
2.01 |
| Chamber saturation time: 15 min |
2.12 ± 55 |
2.59 |
| Solvent migration distance: 78 mm |
2.12 ± 53 |
2.51 |
| Solvent migration distance: 80 mm |
2.12 ± 43 |
2.01 |
| Solvent migration distance: 82 mm |
2.12 ± 39 |
1.84 |
| (B) Barlerin |
| Mobile phase composition 3:3.1:2.9:1 |
0.56 |
0.56 ± 15.1 |
2.72 |
| Mobile phase composition 3:3:3:1 |
0.56 ± 9.4 |
1.69 |
| Mobile phase composition 3.2:2.8:3:1 |
0.55 ± 9.6 |
1.74 |
| Mobile phase volume 8 ml |
0.56 ± 7.9 |
1.43 |
| Mobile phase volume 10 ml |
0.55 ± 9.4 |
1.69 |
| Mobile phase volume 12 ml |
0.56 ± 15.5 |
2.80 |
| Chamber saturation time: 5 min |
0.55 ± 11.6 |
2.11 |
| Chamber saturation time: 10 min |
0.56 ± 9.4 |
1.69 |
| Chamber saturation time: 15 min |
0.56 ± 14.2 |
2.56 |
| Solvent migration distance: 78 mm |
0.56 ± 11.1 |
2.00 |
| Solvent migration distance: 80 mm |
0.55 ± 9.4 |
1.69 |
| Solvent migration distance: 82 mm |
0.55 ± 10.8 |
1.95 |
| (C) Shanzhiside methyl ester |
| Mobile phase composition 3:3.1:2.9:1 |
1.20 |
1.19 ± 32 |
2.71 |
| Mobile phase composition 3:3:3:1 |
1.20 ± 18 |
1.54 |
| Mobile phase composition 3.2:2.8:3:1 |
1.19 ± 38 |
3.14 |
| Mobile phase volume 8 ml |
1.20 ± 38 |
3.16 |
| Mobile phase volume 10 ml |
1.20 ± 18 |
1.54 |
| Mobile phase volume 12 ml |
1.19 ± 26 |
2.16 |
| Chamber saturation time: 5 min |
1.20 ± 29 |
2.38 |
| Chamber saturation time: 10 min |
1.20 ± 18 |
1.54 |
| Chamber saturation time: 15 min |
1.19 ± 39 |
3.21 |
| Solvent migration distance: 78 mm |
1.20 ± 34 |
2.82 |
| Solvent migration distance: 80 mm |
1.20 ± 18 |
1.54 |
| Solvent migration distance: 82 mm |
1.19 ± 37 |
3.11 |
