| Amino Acid |
LOQ (µg mL-1) |
LOD (µg mL-1) |
Retention time (min) |
Linear calibration range |
Coefficient of determination (r2) |
Linear regression equation |
| Gly |
0.18 |
0.06 |
3 |
1-10 |
0.9983 |
y = 4.53x + 0.02 |
| Ala |
0.18 |
0.06 |
3.2 |
1-10 |
0.9999 |
y = 4.405x + 0.17 |
| 1,3 Dap |
0.18 |
0.06 |
3.5 |
1-10 |
0.9962 |
y = 3.8357x + 0.0558 |
| Put |
0.18 |
0.06 |
3.8 |
1-10 |
0.9995 |
y = 3.6979x + 0.2659 |
| Ser |
0.31 |
0.1 |
4 |
1-10 |
0.9992 |
y = 4.145x - 0.05 |
| Cad |
0.18 |
0.06 |
4.2 |
1-10 |
0.9984 |
y = 3.9778x + 0.0793 |
| Pro |
0.31 |
0.1 |
4.6 |
1-10 |
0.9933 |
y = 3.41x + 0.24 |
| GABA |
0.18 |
0.06 |
4.8 |
1-10 |
0.9991 |
y = 4.5704x + 0.2958 |
| Val |
0.31 |
0.1 |
5 |
1-10 |
0.9912 |
y = 4.975x + 0.45 |
| Thr |
0.31 |
0.1 |
5.3 |
1-10 |
0.9946 |
y = 3.92x + 0.34 |
| Leu |
0.61 |
0.2 |
5.5 |
1-10 |
0.9959 |
y = 4.765x + 0.11 |
| Ile |
0.61 |
0.2 |
5.8 |
1-10 |
0.9993 |
y = 4.64x + 0.02 |
| Cys |
0.18 |
0.06 |
6 |
1-10 |
0.9978 |
y = 3.525x - 0.03 |
| Asn |
0.18 |
0.06 |
6.2 |
1-10 |
0.9998 |
y = 3.235x + 0.17 |
| Asp |
0.18 |
0.06 |
6.5 |
1-10 |
0.9965 |
y = 2.835x + 1.21 |
| Hst |
0.31 |
0.1 |
6.8 |
1-10 |
0.9945 |
y = 4.3707x - 0.6397 |
| Lys |
0.31 |
0.1 |
7 |
1-10 |
0.9919 |
y = 3.65x - 0.2 |
| Gln |
0.31 |
0.1 |
7.3 |
1-10 |
0.9992 |
y = 3.72x + 0.02 |
| Glu |
0.18 |
0.06 |
7.6 |
1-10 |
0.9999 |
y = 3.03x + 0.1 |
| Met |
0.61 |
0.2 |
8 |
1-10 |
0.9968 |
y = 4.23x + 0.04 |
| Spd |
0.31 |
0.1 |
8.2 |
1-10 |
0.9953 |
y = 4.1267x + 0.49 |
| His |
0.61 |
0.2 |
8.6 |
1-10 |
0.9966 |
y = 4.305x + 0.07 |
| Phe |
0.31 |
0.1 |
9.1 |
1-10 |
0.9987 |
y = 3.825x - 0.01 |
| Dop |
0.61 |
0.2 |
9.4 |
1-10 |
0.9933 |
y = 4.3824x + 0.069 |
| Arg |
0.31 |
0.1 |
9.7 |
1-10 |
0.998 |
y = 4.01x + 0.54 |
| Tyr |
0.31 |
0.1 |
10.2 |
1-10 |
0.9995 |
y = 3.325x + 0.07 |
| Trp |
0.61 |
0.2 |
10.6 |
1-10 |
0.9977 |
y = 4.85x + 0.62 |
| Spm |
0.61 |
0.2 |
11 |
1-10 |
0.9949 |
y = 4.5971x + 0.9761 |
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