Figure 1: HPTLC image of Bupleuri radix (Chai-Hu)*. (A) HPTLC fluorescence image (365nm); (B) HPTLC visible color image; (C) HPTLC fluorescence-quenching image (254 nm), (saikosaponin b2 can be detected under UV 254nm as a fluorescence-quenching band). (S1): saikosaponin f; (S2) saikosaponin b2; (S3) saikosaponin a; (S4) saikosaponin d; (1)(2)(5): Bupleurum chinese, (3) (6):B. scorzonerifolium, (4) B. falcatum, (7) B. longiradiatum, (8) B.marginatum, (9) B. poly clonum, (10) B. wenchuanese, (11) B. marginatum var. stenophyllum, (12) B. falcatum, (13) B. yinchowense, (14) B. simithii var. parvifolium, (15) B. tenue.