Figure 10: Partial detail of HPTLC image of the roots of two species of Astragalus (Huang-Qi) to show distinction between the two species: AMM = Astragalus membranaceus var. mongholicus; AM = Astragalus membranaceus (S3) calyosin-7-O-glucoside; (So) ononin. A: HPTLC fluorescence images of (L) Astragulus membranaceus (AM) and (R) A. membranaceus var. mongholicus (AMM) B: Partial images of (A): The partial fraction of (A): AMM showed distinct light greyish-green fluorescence band of ononin (So), and greyish blue fluorescence band of clyosin-7-glucoside (S3); but AM showed two obvious light orange fluorescence bands of saponins (*), (So) and (S3) are very weak. C: The invert color of image transferred from image (B) for clearer discern. AMM: the band (So) turned to dark purple color, (S3) turned to weak brownish yellow; AM: two saponin bands (*) turn to greyish blue color, (So) and (S3) are shallow. D: The fluorescence-quenching image on silica gel 254 plate. The distinct fluorescence quenching bands of calyosin-7-glucoside (S3) and ononin (So) under UV 254 light in AMM are obvious but hard observed in AM.