| Chinese herbal drug |
Sample solution |
Developing solvent system |
Visualization |
| Bupleuri radix (Chai Hu) [34,35] |
ethanol extract (containing trace amount pyridine) – C18 cartridge clean-up – 80% eluate – methanol solution |
dichloromethane-acetyl acetate- methanol-water (30：40：15：3) |
Dimethylamino-benzaldehyde (DMAB) (2%) in sulfuric acid alcohol solution (10%), heat at 105℃ for 1 min, observe fluorescence image |
| Forsythiaefructus(LianQiao)
Unpublished data |
ultrasonicate with acetone – methanol solution |
(1) for higher polarity fraction::chloroform – ethyl acetate – methanol – formic acid (30 : 5 : 10 : 1)
(2) for lower polarity fraction: Toluene – ethyl acetate – 36% acetic acid (90 : 25 : 2) |
10% sulphuric acid in ethanol, heated at 105°Cuntil the bands color are distinct enough, examine the visible color images under day light and the fluorescence images under UV light at 366 nm. |
| Rehmanniae radix(Di-Huang) [37] |
ultrosonicate at 40℃ - centrifuge for 10 minutes (4000 r/min), use the supernatant as sample solution. |
dichloromethane-ethyl acetate-methanol-water-glacial acetic acid (8:4:7:1:1) |
2% anisaldehyde in 5% sulphuric acid ethanol solution, heat at 105℃until the color of the bands are clearly visible. |
| Salviaemiltiorrhizae radix (Dan Shen)
Unpublished data |
•Lipophilic fraction: ultrasonicate with ethyl acetate extract – ethyl acetate solution;
•Hydrophilic fraction: ultrasonic extract at 60℃with water, the filtrate added small amount 10% HCl, extracted by ethyl acetate – ethyl acetate solution
|
•Lipophilic fraction: n-Hexane - ethyl acetate - formic acid (30：10：0.5);
•Hydrophilic fraction: toluene- ethyl acetate – methanol – formic acid(5：4：0.5 : 2)
|
•Lipophilic fraction: visible color image under day light; fluorescence-quenching image under UV 254 nm light
•Hydrophilic fraction: 5%vanillin/conc. H2SO4reagent; visible color image under day light;
|
